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Applied and Environmental Microbiology
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gene regulation

  • A CRISPRi-dCas9 System for Archaea and Its Use To Examine Gene Function during Nitrogen Fixation by <span class="named-content genus-species" id="named-content-1">Methanosarcina acetivorans</span>
    Methods
    A CRISPRi-dCas9 System for Archaea and Its Use To Examine Gene Function during Nitrogen Fixation by Methanosarcina acetivorans

    Genetic tools are needed to understand and manipulate the biology of archaea, which serve critical roles in the biosphere. Methanogenic archaea (methanogens) are essential for the biological production of methane, an intermediate in the global carbon cycle, an important greenhouse gas, and a biofuel. The CRISPRi-dCas9 system in the model methanogen Methanosarcina acetivorans...

    Ahmed E. Dhamad, Daniel J. Lessner
  • Fungal-Associated Molecules Induce Key Genes Involved in the Biosynthesis of the Antifungal Secondary Metabolites Nunamycin and Nunapeptin in the Biocontrol Strain <span class="named-content genus-species" id="named-content-1">Pseudomonas fluorescens</span> In5
    Environmental Microbiology
    Fungal-Associated Molecules Induce Key Genes Involved in the Biosynthesis of the Antifungal Secondary Metabolites Nunamycin and Nunapeptin in the Biocontrol Strain Pseudomonas fluorescens In5

    Cyclic lipopeptide (CLP) synthesis gene clusters in pseudomonads display a high degree of synteny, and the structures of the peptides synthesized are very similar. Accordingly, the genomic island encoding the synthesis of syringomycin and syringopeptin in P. syringae pv. syringae closely resembles that of...

    Line Christiansen, Katrine Skov Alanin, Christopher B. W. Phippen, Stefan Olsson, Peter Stougaard, Rosanna C. Hennessy
  • Open Access
    LuxR Solos in the Plant Endophyte <em>Kosakonia</em> sp. Strain KO348
    Environmental Microbiology
    LuxR Solos in the Plant Endophyte Kosakonia sp. Strain KO348

    Cell-cell signaling in bacteria allows a synchronized and coordinated behavior of a microbial community. LuxR solos represent a subfamily of proteins in proteobacteria which most commonly detect and respond to signals produced exogenously by other microbes or eukaryotic hosts. Here, we report that a plant-beneficial bacterial endophyte belonging to the novel genus of Kosakonia possesses two LuxR solos; one is involved in the...

    Susan Mosquito, Xianfa Meng, Giulia Devescovi, Iris Bertani, Alexander M. Geller, Asaf Levy, Michael P. Myers, Cristina Bez, Sonia Covaceuszach, Vittorio Venturi
  • Open Access
    Wood Modification by Furfuryl Alcohol Resulted in a Delayed Decomposition Response in <em>Rhodonia</em> (<em>Postia</em>) <em>placenta</em>
    Biodegradation
    Wood Modification by Furfuryl Alcohol Resulted in a Delayed Decomposition Response in Rhodonia (Postia) placenta

    Fungi are important decomposers of woody biomass in natural habitats. Investigation of the mechanisms employed by decay fungi in their attempt to degrade wood is important for both the basic scientific understanding of ecology and carbon cycling in nature and for applied uses of woody materials. For wooden building materials, long service life and carbon storage are essential, but decay fungi are responsible for massive losses of wood...

    Inger Skrede, Monica Hongrø Solbakken, Jaqueline Hess, Carl Gunnar Fossdal, Olav Hegnar, Gry Alfredsen
  • Open Access
    Development of Aspirin-Inducible Biosensors in <em>Escherichia coli</em> and SimCells
    Genetics and Molecular Biology | Spotlight
    Development of Aspirin-Inducible Biosensors in Escherichia coli and SimCells

    An aspirin-inducible SalR/Psal regulation system, originally from Acinetobacter baylyi ADP1, has been designed for E. coli strains. SalR is a typical LysR-type transcriptional regulator (LTTR) family protein and activates the Psal promoter in the presence of...

    Jack Xiaoyu Chen, Harrison Steel, Yin-Hu Wu, Yun Wang, Jiabao Xu, Cordelia P. N. Rampley, Ian P. Thompson, Antonis Papachristodoulou, Wei E. Huang
  • Open Access
    Genetics and Molecular Biology
    Promoter Engineering Reveals the Importance of Heptameric Direct Repeats for DNA Binding by Streptomyces Antibiotic Regulatory Protein–Large ATP-Binding Regulator of the LuxR Family (SARP-LAL) Regulators in Streptomyces natalensis
    Eva G. Barreales, Cláudia M. Vicente, Antonio de Pedro, Javier Santos-Aberturas, Jesús F. Aparicio
  • Genetics and Molecular Biology
    The 14-3-3 Protein Homolog ArtA Regulates Development and Secondary Metabolism in the Opportunistic Plant Pathogen Aspergillus flavus
    Beatriz A. Ibarra, Jessica M. Lohmar, Timothy Satterlee, Taylor McDonald, Jeffrey W. Cary, Ana M. Calvo
  • Genetics and Molecular Biology
    Coordinated Regulation of the EIIMan and fruRKI Operons of Streptococcus mutans by Global and Fructose-Specific Pathways
    Lin Zeng, Brinta Chakraborty, Tanaz Farivar, Robert A. Burne
  • Genetics and Molecular Biology
    A Genome-Wide Search for Ionizing-Radiation-Responsive Elements in Deinococcus radiodurans Reveals a Regulatory Role for the DNA Gyrase Subunit A Gene's 5′ Untranslated Region in the Radiation and Desiccation Response
    Jordan K. Villa, Paul Amador, Justin Janovsky, Arijit Bhuyan, Roland Saldanha, Thomas J. Lamkin, Lydia M. Contreras
  • Genetics and Molecular Biology
    Revisiting the Regulation of the Primary Scaffoldin Gene in Clostridium thermocellum
    Lizett Ortiz de Ora, Iván Muñoz-Gutiérrez, Edward A. Bayer, Yuval Shoham, Raphael Lamed, Ilya Borovok

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