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Applied and Environmental Microbiology
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genetics

  • Open Access
    Creation of Universal Primers Targeting Nonconserved, Horizontally Mobile Genes: Lessons and Considerations
    Genetics and Molecular Biology
    Creation of Universal Primers Targeting Nonconserved, Horizontally Mobile Genes: Lessons and Considerations

    Increasing use of molecular detection methods, specifically PCR and quantitative PCR (qPCR), requires utmost confidence in the results while minimizing false positives and negatives due to poor primer designs. Frequently, these detection methods are focused on conserved core genes, which limits their applications.

    Damon C. Brown, Raymond J. Turner
  • Antibacterial Activity of <em>Bacillus inaquosorum</em> Strain T1 against <em>pirAB<sup>Vp</sup></em>-Bearing <span class="named-content genus-species" id="named-content-1">Vibrio parahaemolyticus</span>: Genetic and Physiological Characterization
    Genetics and Molecular Biology
    Antibacterial Activity of Bacillus inaquosorum Strain T1 against pirABVp-Bearing Vibrio parahaemolyticus: Genetic and Physiological Characterization

    The shrimp aquaculture industry has been significantly impacted by acute hepatopancreatic necrosis disease (AHPND), resulting in significant financial losses annually. AHPND is caused by strains of the bacterial pathogen Vibrio parahaemolyticus, and treatment of AHPND involves the use of antibiotics, which leads to a rise in the number of antibiotic-resistant strains...

    Sarah E. Avery, Susannah P. Ruzbarsky, Amanda M. Hise, Harold J. Schreier
  • Genetic Determinants of <em>Salmonella</em> Resistance to the Biofilm-Inhibitory Effects of a Synthetic 4-Oxazolidinone Analog
    Genetics and Molecular Biology
    Genetic Determinants of Salmonella Resistance to the Biofilm-Inhibitory Effects of a Synthetic 4-Oxazolidinone Analog

    Biofilms are resistant to killing by disinfectants and antimicrobials. S. enterica biofilms facilitate long-term host colonization and persistence in food processing environments. Synthetic analogs of 4-oxazolidinone natural products show promise as antibiofilm agents. Here, we show that a synthetic 4-oxazolidinone analog inhibits Salmonella biofilm through...

    K. F. Griewisch, J. G. Pierce, J. R. Elfenbein
  • Stable Transformation of the Actinobacteria <em>Frankia</em> spp.
    Plant Microbiology | Spotlight
    Stable Transformation of the Actinobacteria Frankia spp.

    The absence of genetic tools for Frankia research has been a major hindrance to the associated field of actinorhizal symbiosis and the use of the nitrogen-fixing actinobacteria. This study reports on the introduction of plasmids into Frankia spp. and their functional expression of green fluorescent protein and a cloned gene. As the first step in developing genetic tools, this technique opens up the field to a wide...

    Céline Pesce, Rediet Oshone, Sheldon G. Hurst, Victoria A. Kleiner, Louis S. Tisa
  • Rapid, Parallel Identification of Catabolism Pathways of Lignin-Derived Aromatic Compounds in <span class="named-content genus-species" id="named-content-1">Novosphingobium aromaticivorans</span>
    Biodegradation | Spotlight
    Rapid, Parallel Identification of Catabolism Pathways of Lignin-Derived Aromatic Compounds in Novosphingobium aromaticivorans

    The identification of the genes involved in specific biochemical transformations is a key step in predicting microbial function from nucleic acid sequences and in engineering microbes to endow them with new functions. We have shown that new techniques for transposon mutagenesis can dramatically simplify this process and enable the rapid identification of genes in uncharacterized pathways. These techniques provide the necessary scale to...

    Jacob H. Cecil, David C. Garcia, Richard J. Giannone, Joshua K. Michener
  • Methods
    Tools for Rapid Genetic Engineering of Vibrio fischeri
    Karen L. Visick, Kelsey M. Hodge-Hanson, Alice H. Tischler, Allison K. Bennett, Vincent Mastrodomenico
  • Genetics and Molecular Biology
    Microhomology-Mediated High-Throughput Gene Inactivation Strategy for the Hyperthermophilic Crenarchaeon Sulfolobus islandicus
    Changyi Zhang, Rachel J. Whitaker
  • Invertebrate Microbiology
    A Third Type of Resistance to Cydia pomonella Granulovirus in Codling Moths Shows a Mixed Z-Linked and Autosomal Inheritance Pattern
    A. J. Sauer, S. Schulze-Bopp, E. Fritsch, K. Undorf-Spahn, J. A. Jehle
  • Environmental Microbiology
    Arsenic Detoxification by Geobacter Species
    Yan Dang, David J. F. Walker, Kaitlin E. Vautour, Steven Dixon, Dawn E. Holmes
  • Biotechnology
    Development of a High-Efficiency Transformation Method and Implementation of Rational Metabolic Engineering for the Industrial Butanol Hyperproducer Clostridium saccharoperbutylacetonicum Strain N1-4
    Nicolaus A. Herman, Jeffrey Li, Ripika Bedi, Barbara Turchi, Xiaoji Liu, Michael J. Miller, Wenjun Zhang
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