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genetics

  • Stable Transformation of the Actinobacteria <em>Frankia</em> spp.
    Plant Microbiology | Spotlight
    Stable Transformation of the Actinobacteria Frankia spp.

    The absence of genetic tools for Frankia research has been a major hindrance to the associated field of actinorhizal symbiosis and the use of the nitrogen-fixing actinobacteria. This study reports on the introduction of plasmids into Frankia spp. and their functional expression of green fluorescent protein and a cloned gene. As the first step in developing genetic tools, this technique opens up the field to a wide...

    Céline Pesce, Rediet Oshone, Sheldon G. Hurst, Victoria A. Kleiner, Louis S. Tisa
  • Rapid, Parallel Identification of Catabolism Pathways of Lignin-Derived Aromatic Compounds in <span class="named-content genus-species" id="named-content-1">Novosphingobium aromaticivorans</span>
    Biodegradation | Spotlight
    Rapid, Parallel Identification of Catabolism Pathways of Lignin-Derived Aromatic Compounds in Novosphingobium aromaticivorans

    The identification of the genes involved in specific biochemical transformations is a key step in predicting microbial function from nucleic acid sequences and in engineering microbes to endow them with new functions. We have shown that new techniques for transposon mutagenesis can dramatically simplify this process and enable the rapid identification of genes in uncharacterized pathways. These techniques provide the necessary scale to...

    Jacob H. Cecil, David C. Garcia, Richard J. Giannone, Joshua K. Michener
  • Methods
    Tools for Rapid Genetic Engineering of Vibrio fischeri
    Karen L. Visick, Kelsey M. Hodge-Hanson, Alice H. Tischler, Allison K. Bennett, Vincent Mastrodomenico
  • Genetics and Molecular Biology
    Microhomology-Mediated High-Throughput Gene Inactivation Strategy for the Hyperthermophilic Crenarchaeon Sulfolobus islandicus
    Changyi Zhang, Rachel J. Whitaker
  • Invertebrate Microbiology
    A Third Type of Resistance to Cydia pomonella Granulovirus in Codling Moths Shows a Mixed Z-Linked and Autosomal Inheritance Pattern
    A. J. Sauer, S. Schulze-Bopp, E. Fritsch, K. Undorf-Spahn, J. A. Jehle
  • Environmental Microbiology
    Arsenic Detoxification by Geobacter Species
    Yan Dang, David J. F. Walker, Kaitlin E. Vautour, Steven Dixon, Dawn E. Holmes
  • Biotechnology
    Development of a High-Efficiency Transformation Method and Implementation of Rational Metabolic Engineering for the Industrial Butanol Hyperproducer Clostridium saccharoperbutylacetonicum Strain N1-4
    Nicolaus A. Herman, Jeffrey Li, Ripika Bedi, Barbara Turchi, Xiaoji Liu, Michael J. Miller, Wenjun Zhang
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Print ISSN: 0099-2240; Online ISSN: 1098-5336