Genetics, Microbial

Genetics and Molecular Biology
Use of Natural Transformation To Establish an Easy Knockout Method in Riemerella anatipestifer
MaFeng Liu, Li Zhang, Li Huang, Francis Biville, DeKang Zhu, MingShu Wang, RenYong Jia, Shun Chen, KunFeng Sun, Qiao Yang, Ying Wu, XiaoYue Chen, AnChun Cheng
Mycology
Agrobacterium tumefaciens-Mediated Transformation for Investigation of Somatic Recombination in the Fungal Pathogen Armillaria mellea
Kendra Baumgartner, Phillip Fujiyoshi, Gary D. Foster, Andy M. Bailey
Methods
Expanding the Genetic Toolbox for Leptospira Species by Generation of Fluorescent Bacteria
Florence Aviat, Leyla Slamti, Gustavo M. Cerqueira, Kristel Lourdault, Mathieu Picardeau
Genetics and Molecular Biology
Development of a Versatile Procedure Based on Natural Transformation for Marker-Free Targeted Genetic Modification in Streptococcus thermophilus
Laetitia Fontaine, Damien Dandoy, Céline Boutry, Brigitte Delplace, Marie Henry de Frahan, Christophe Fremaux, Philippe Horvath, Patrick Boyaval, Pascal Hols
Methods
Synthetic Riboswitches That Induce Gene Expression in Diverse Bacterial Species
Shana Topp, Colleen M. K. Reynoso, Jessica C. Seeliger, Ian S. Goldlust, Shawn K. Desai, Dorothée Murat, Aimee Shen, Aaron W. Puri, Arash Komeili, Carolyn R. Bertozzi, June R. Scott, Justin P. Gallivan
Genetics and Molecular Biology
Genetic Transformation and Mutagenesis via Single-Stranded DNA in the Unicellular, Diazotrophic Cyanobacteria of the Genus Cyanothece
Hongtao Min, Louis A. Sherman
Methods
lacZ Reporter System for Use in Borrelia burgdorferi
Beth M. Hayes, Mollie W. Jewett, Patricia A. Rosa
Methods
Targeted Chromosomal Knockouts in Mycoplasma pneumoniae
Radha Krishnakumar, Nacyra Assad-Garcia, Gwynedd A. Benders, Quang Phan, Michael G. Montague, John I. Glass
Methods
Xer Site-Specific Recombination, an Efficient Tool To Introduce Unmarked Deletions into Mycobacteria
Alessandro Cascioferro, Francesca Boldrin, Agnese Serafini, Roberta Provvedi, Giorgio Palù, Riccardo Manganelli
Methods
Simple Genetic Selection Protocol for Isolation of Overexpressed Genes That Enhance Accumulation of Membrane-Integrated Human G Protein-Coupled Receptors in Escherichia coli
Georgios Skretas, George Georgiou

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