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HTS

  • Primer Design for an Accurate View of Picocyanobacterial Community Structure by Using High-Throughput Sequencing
    Microbial Ecology
    Primer Design for an Accurate View of Picocyanobacterial Community Structure by Using High-Throughput Sequencing

    The molecular diversity of the aquatic picocyanobacterial community cannot be accurately described using only the available universal 16S rRNA gene primers that target the whole bacterial and archaeal community. We show that the hypervariable regions V5, V6, and V7 of the 16S rRNA gene are better suited to study the diversity, community structure, and dynamics of picocyanobacterial communities at a fine scale using Illumina MiSeq...

    Paula Huber, Francisco M. Cornejo-Castillo, Isabel Ferrera, Pablo Sánchez, Ramiro Logares, Sebastián Metz, Vanessa Balagué, Silvia G. Acinas, Josep M. Gasol, Fernando Unrein
  • A Diverse Range of Human Gut Bacteria Have the Potential To Metabolize the Dietary Component Gallic Acid
    Food Microbiology
    A Diverse Range of Human Gut Bacteria Have the Potential To Metabolize the Dietary Component Gallic Acid

    Despite the increasing wealth of sequencing data, the health contributions of many bacteria found in the human gut microbiota have yet to be elucidated. This study applies a novel experimental approach to predict the ability of gut microbes to carry out a specific metabolic activity, i.e., gallate metabolism. The study showed that, while gallate-decarboxylating bacteria represented 47% of the bacterial genera detected previously in the...

    María Esteban-Torres, Laura Santamaría, Raúl Cabrera-Rubio, Laura Plaza-Vinuesa, Fiona Crispie, Blanca de las Rivas, Paul Cotter, Rosario Muñoz
  • Dynamics and Biodiversity of Bacterial and Yeast Communities during Fermentation of Cocoa Beans
    Food Microbiology
    Dynamics and Biodiversity of Bacterial and Yeast Communities during Fermentation of Cocoa Beans

    In spite of the limited effectiveness of the considered inoculated starter strains, this study provides new information on the microbial development of box and heap cocoa fermentations, under inoculated and noninoculated conditions, as we coupled yeast/bacterial amplicon-based sequencing data with microbial metabolite detection. The information so far available suggests that microbial communities have played an important role in the...

    Jatziri Mota-Gutierrez, Cristian Botta, Ilario Ferrocino, Manuela Giordano, Marta Bertolino, Paola Dolci, Marcella Cannoni, Luca Cocolin
  • Open Access
    Environmental Microbiology
    Assessing Performance of Spore Samplers in Monitoring Aeromycobiota and Fungal Plant Pathogen Diversity in Canada
    Wen Chen, Sarah Hambleton, Keith A. Seifert, Odile Carisse, Moussa S. Diarra, Rick D. Peters, Christine Lowe, Julie T. Chapados, C. André Lévesque
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Print ISSN: 0099-2240; Online ISSN: 1098-5336