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Applied and Environmental Microbiology
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marine microbiome

  • Towards Quantitative Microbiome Community Profiling Using Internal Standards
    Methods
    Towards Quantitative Microbiome Community Profiling Using Internal Standards

    High-throughput-sequencing-based marine microbiome profiling is rapidly expanding and changing how we study the oceans. Although powerful, the technique is not fully quantitative; it provides taxon counts only in relative abundances. In order to address this issue, we present a method to quantitatively estimate microbial abundances per unit volume of seawater filtered by spiking known amounts of internal DNA standards into each sample....

    Yajuan Lin, Scott Gifford, Hugh Ducklow, Oscar Schofield, Nicolas Cassar
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Print ISSN: 0099-2240; Online ISSN: 1098-5336