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metabolic engineering

  • Anaerobic Production of Isoprene by Engineered <em>Methanosarcina</em> Species Archaea
    Physiology
    Anaerobic Production of Isoprene by Engineered Methanosarcina Species Archaea

    A significant barrier to implementing renewable chemical technologies is high production costs relative to those for petroleum-derived products. Existing technologies using engineered organisms have difficulty competing with petroleum-derived chemicals due to the cost of feedstocks (such as glucose), product extraction, and purification.

    Jared Aldridge, Sean Carr, Karrie A. Weber, Nicole R. Buan
  • Diamine Biosynthesis: Research Progress and Application Prospects
    Minireview
    Diamine Biosynthesis: Research Progress and Application Prospects

    Diamines are important monomers for polyamide plastics; they include 1,3-diaminopropane, 1,4-diaminobutane, 1,5-diaminopentane, and 1,6-diaminohexane, among others. With increasing attention on environmental problems and green sustainable development, utilizing renewable raw materials for the synthesis of diamines is crucial for the establishment of a sustainable plastics industry. Recently, high-performance microbial factories, such as...

    Li Wang, Guohui Li, Yu Deng
  • Open Access
    Multiplex Design of the Metabolic Network for Production of <span class="sc">l</span>-Homoserine in <span class="named-content genus-species" id="named-content-1">Escherichia coli</span>
    Biotechnology | Spotlight
    Multiplex Design of the Metabolic Network for Production of l-Homoserine in Escherichia coli

    In this study, the bottlenecks that sequentially limit l-homoserine biosynthesis were identified and resolved, based on rational and efficient metabolic-engineering strategies, coupled with CRISPR interference (CRISPRi)-based systematic analysis. The metabolomics data largely expanded our understanding of metabolic effects and revealed relevant targets for further modification to achieve better performance. The...

    Peng Liu, Bo Zhang, Zhen-Hao Yao, Zhi-Qiang Liu, Yu-Guo Zheng
  • Engineering a Carotenoid-Overproducing Strain of <span class="named-content genus-species" id="named-content-1">Azospirillum brasilense</span> for Heterologous Production of Geraniol and Amorphadiene
    Biotechnology
    Engineering a Carotenoid-Overproducing Strain of Azospirillum brasilense for Heterologous Production of Geraniol and Amorphadiene

    To date, the major host organisms used for the heterologous production of terpenoids, i.e., E. coli and S. cerevisiae, do not have high-flux isoprenoid pathways and involve tedious metabolic engineering to increase the precursor pool. Since carotenoid-producing bacteria carry endogenous high-flux...

    Shivangi Mishra, Parul Pandey, Ashutosh Prakash Dubey, Aafreen Zehra, Chandan Singh Chanotiya, Anil Kumar Tripathi, Mukti Nath Mishra
  • A CRISPR/Anti-CRISPR Genome Editing Approach Underlines the Synergy of Butanol Dehydrogenases in <span class="named-content genus-species" id="named-content-1">Clostridium acetobutylicum</span> DSM 792
    Genetics and Molecular Biology
    A CRISPR/Anti-CRISPR Genome Editing Approach Underlines the Synergy of Butanol Dehydrogenases in Clostridium acetobutylicum DSM 792

    An efficient CRISPR-Cas9 editing tool based on a previous two-plasmid system was developed for Clostridium acetobutylicum and used to investigate the contribution of chromosomal butanol dehydrogenase genes during solventogenesis. Thanks to the control of cas9 expression by inducible promoters and of Cas9-guide RNA (gRNA) complex activity by an anti-CRISPR...

    François Wasels, Gwladys Chartier, Rémi Hocq, Nicolas Lopes Ferreira
  • Open Access
    Exploiting the Diversity of Saccharomycotina Yeasts To Engineer Biotin-Independent Growth of <span class="named-content genus-species" id="named-content-1">Saccharomyces cerevisiae</span>
    Biotechnology | Spotlight
    Exploiting the Diversity of Saccharomycotina Yeasts To Engineer Biotin-Independent Growth of Saccharomyces cerevisiae

    The reported metabolic engineering strategy to enable optimal growth in the absence of biotin is of direct relevance for large-scale industrial applications of S. cerevisiae. Important benefits of biotin prototrophy include cost reduction during the preparation of chemically defined industrial growth media as well as a lower susceptibility of biotin-prototrophic...

    Anna K. Wronska, Meinske P. Haak, Ellen Geraats, Eva Bruins Slot, Marcel van den Broek, Jack T. Pronk, Jean-Marc Daran
  • Combining Random Mutagenesis and Metabolic Engineering for Enhanced Tryptophan Production in <em>Synechocystis</em> sp. Strain PCC 6803
    Biotechnology
    Combining Random Mutagenesis and Metabolic Engineering for Enhanced Tryptophan Production in Synechocystis sp. Strain PCC 6803

    Aromatic amino acids such as tryptophan are primarily used as additives in the animal feed industry and are typically produced using genetically engineered heterotrophic organisms such as Escherichia coli. This involves a two-step process, where the substrate such as molasses is first obtained from plants followed by fermentation by heterotrophic organisms. We have...

    Arnav Deshpande, Jeremiah Vue, John Morgan
  • Multistep Metabolic Engineering of <span class="named-content genus-species" id="named-content-1">Bacillus licheniformis</span> To Improve Pulcherriminic Acid Production
    Biotechnology
    Multistep Metabolic Engineering of Bacillus licheniformis To Improve Pulcherriminic Acid Production

    Pulcherriminic acid is a cyclodipeptide derived from cyclo(l-Leu–l-Leu), which shares the same iron chelation group with hydroxamate sidephores. Generally, pulcherriminic acid-producing strains could be the perfect candidates for antibacterial and anti-plant-pathogenic fungal agents. In this study, we obtained the promising W4/pHY-yvmA pulcherriminic acid-producing strain via a...

    Shiyi Wang, Huan Wang, Dan Zhang, Xiaoyun Li, Jiang Zhu, Yangyang Zhan, Dongbo Cai, Qin Wang, Xin Ma, Dong Wang, Shouwen Chen
  • Mechanism of Tolerance to the Lignin-Derived Inhibitor <em>p</em>-Benzoquinone and Metabolic Modification of Biorefinery Fermentation Strains
    Biotechnology
    Mechanism of Tolerance to the Lignin-Derived Inhibitor p-Benzoquinone and Metabolic Modification of Biorefinery Fermentation Strains

    This study advances our understanding of BQ inhibition behavior and the mechanism of microbial tolerance to this inhibitor and identifies the key genes responsible for BQ detoxification. The insights here into BQ toxicity and tolerance provide the basis for future synthetic biology to engineer industrial fermentation strains with enhanced BQ tolerance.

    Zhao Yan, Xiaochuang Gao, Qiuqiang Gao, Jie Bao
  • CRISPR/Cas9-Assisted Seamless Genome Editing in <span class="named-content genus-species" id="named-content-1">Lactobacillus plantarum</span> and Its Application in <em>N</em>-Acetylglucosamine Production
    Biotechnology
    CRISPR/Cas9-Assisted Seamless Genome Editing in Lactobacillus plantarum and Its Application in N-Acetylglucosamine Production

    CRISPR/Cas9-assisted recombineering is restricted in lactic acid bacteria because of the lack of available antibiotics and vectors. In this study, a seamless genome editing method was carried out in Lactobacillus plantarum using CRISPR/Cas9-assisted double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA) recombineering, and recombination efficiency was effectively...

    Ding Zhou, Zhennan Jiang, Qingxiao Pang, Yuan Zhu, Qian Wang, Qingsheng Qi

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