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Applied and Environmental Microbiology
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metagenomes

  • The Reliability of Metagenome-Assembled Genomes (MAGs) in Representing Natural Populations: Insights from Comparing MAGs against Isolate Genomes Derived from the Same Fecal Sample
    Microbial Ecology
    The Reliability of Metagenome-Assembled Genomes (MAGs) in Representing Natural Populations: Insights from Comparing MAGs against Isolate Genomes Derived from the Same Fecal Sample

    Metagenome assembly and the recovery of metagenome-assembled genomes (MAGs) have recently become common tasks for microbiome studies across environmental and clinical settings. However, the extent to which MAGs can capture the genes of the population they represent remains speculative.

    Alexandra Meziti, Luis M. Rodriguez-R, Janet K. Hatt, Angela Peña-Gonzalez, Karen Levy, Konstantinos T. Konstantinidis
  • Nitrate Consumers in Arctic Marine Eukaryotic Communities: Comparative Diversities of 18S rRNA, 18S rRNA Genes, and Nitrate Reductase Genes
    Environmental Microbiology
    Nitrate Consumers in Arctic Marine Eukaryotic Communities: Comparative Diversities of 18S rRNA, 18S rRNA Genes, and Nitrate Reductase Genes

    To better understand the diversity of primary producers in the Arctic Ocean, we targeted a nitrogen cycle gene, NR, which is required for phytoplankton to assimilate nitrate into organic forms of nitrogen macromolecules. We compared this to the more detailed taxonomy from ice-influenced stations using a general taxonomic gene (18S rRNA). NR genes were ubiquitous and could be classified as belonging to diatoms, dinoflagellates, other...

    André M. Comeau, Marcos G. Lagunas, Karen Scarcella, Diana E. Varela, Connie Lovejoy
  • Open Access
    Biodegradation
    New Insights into the Function and Global Distribution of Polyethylene Terephthalate (PET)-Degrading Bacteria and Enzymes in Marine and Terrestrial Metagenomes
    Dominik Danso, Christel Schmeisser, Jennifer Chow, Wolfgang Zimmermann, Ren Wei, Christian Leggewie, Xiangzhen Li, Terry Hazen, Wolfgang R. Streit
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