mobile genetic elements
Public and Environmental Health MicrobiologyThe Shared Resistome of Human and Pig Microbiota Is Mobilized by Distinct Genetic Elements...There is growing concern that antibiotic resistance genes could spread from the husbandry environment to human pathogens through dissemination mediated by mobile genetic elements. In this study, we investigated the contribution of mobile genetic elements to the abundance of highly prevalent antibiotic resistance genes found in commensal bacteria of both human and pig intestinal microbiota originating from the same region.
Genetics and Molecular BiologyCreation of Universal Primers Targeting Nonconserved, Horizontally Mobile Genes: Lessons and ConsiderationsIncreasing use of molecular detection methods, specifically PCR and quantitative PCR (qPCR), requires utmost confidence in the results while minimizing false positives and negatives due to poor primer designs. Frequently, these detection methods are focused on conserved core genes, which limits their applications.
Environmental MicrobiologyImplications of Foraging and Interspecies Interactions of Birds for Carriage of Escherichia coli Strains Resistant to Critically Important AntimicrobialsIt has been shown that 20% of Australian silver gulls carry drug-resistant Escherichia coli strains of anthropogenic origin associated with severe diseases, such as sepsis and urinary tract infections, in humans. To further characterize the dynamics of drug-resistant E. coli in wildlife populations...
Food MicrobiologyImplications of Mobile Genetic Elements for Salmonella enterica Single-Nucleotide Polymorphism Subtyping and Source Tracking InvestigationsDespite increasingly routine use of WGS and SNP subtyping in outbreak and source tracking investigations, whether and how MGEs affect SNP subtyping has not been thoroughly investigated. Besides chromosomal MGEs, plasmids are frequently entangled in draft genome assemblies and yet to be assessed for their impact on SNP subtyping. This study provides evidence-based guidance on the treatment of MGEs in SNP analysis for Salmonella...
Evolutionary and Genomic MicrobiologyPotentially Mobile Denitrification Genes Identified in Azospirillum sp. Strain TSH58The evolutionary history of denitrification is complex. While the occurrence of horizontal gene transfer has been suggested for denitrification genes, most studies report circumstantial evidences, such as disagreement between denitrification gene phylogenies and the 16S rRNA gene phylogeny. Based on the comparative genome analyses of Azospirillum sp. denitrifiers, we...
- Evolutionary and Genomic Microbiology | SpotlightStaphylococcus edaphicus sp. nov., Isolated in Antarctica, Harbors the mecC Gene and Genomic Islands with a Suspected Role in Adaptation to Extreme Environments
- Genetics and Molecular BiologyGenome Integration and Excision by a New Streptomyces Bacteriophage, ϕJoe