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Applied and Environmental Microbiology
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multidrug resistance

  • Open Access
    Creation of Universal Primers Targeting Nonconserved, Horizontally Mobile Genes: Lessons and Considerations
    Genetics and Molecular Biology
    Creation of Universal Primers Targeting Nonconserved, Horizontally Mobile Genes: Lessons and Considerations

    Increasing use of molecular detection methods, specifically PCR and quantitative PCR (qPCR), requires utmost confidence in the results while minimizing false positives and negatives due to poor primer designs. Frequently, these detection methods are focused on conserved core genes, which limits their applications.

    Damon C. Brown, Raymond J. Turner
  • Identification and Antimicrobial Susceptibility Testing of <em>Campylobacter</em> Using a Microfluidic Lab-on-a-Chip Device
    Methods
    Identification and Antimicrobial Susceptibility Testing of Campylobacter Using a Microfluidic Lab-on-a-Chip Device

    Increasing concerns of antibiotic-resistant Campylobacter spp. with regard to public health emphasize the importance of efficient and fast detection. This study described the timely identification and antimicrobial susceptibility testing of Campylobacter spp. by using a microfluidic device. Our developed method not only reduced the total analysis time, but it also simplified food sample preparation and chip operation...

    Luyao Ma, Marlen Petersen, Xiaonan Lu
  • Open Access
    Loci Encoding Compounds Potentially Active against Drug-Resistant Pathogens amidst a Decreasing Pool of Novel Antibiotics
    Environmental Microbiology
    Loci Encoding Compounds Potentially Active against Drug-Resistant Pathogens amidst a Decreasing Pool of Novel Antibiotics

    Carbapenem-resistant P. aeruginosa is difficult to treat and has been deemed by the World Health Organization as a priority one pathogen for which antibiotics are most urgently needed. Although metagenomics and bioinformatic studies suggest that natural bacteria remain a source of novel compounds, the identification of genes and their products specific to activity...

    Joseph Basalla, Payel Chatterjee, Elizabeth Burgess, Mahnur Khan, Emily Verbrugge, Daniel D. Wiegmann, John J. LiPuma, Hans Wildschutte
  • New Shuttle Vectors for Real-Time Gene Expression Analysis in Multidrug-Resistant <span class="named-content genus-species" id="named-content-1">Acinetobacter</span> Species: <em>In Vitro</em> and <em>In Vivo</em> Responses to Environmental Stressors
    Genetics and Molecular Biology
    New Shuttle Vectors for Real-Time Gene Expression Analysis in Multidrug-Resistant Acinetobacter Species: In Vitro and In Vivo Responses to Environmental Stressors

    The short-term adaptive response to environmental cues greatly contributes to the ecological success of bacteria, and profound alterations in bacterial gene expression occur in response to physical, chemical, and nutritional stresses. Bacteria belonging to the Acinetobacter genus are ubiquitous inhabitants of soil and water though some species, such as...

    Massimiliano Lucidi, Daniela Visaggio, Elisa Prencipe, Francesco Imperi, Giordano Rampioni, Gabriella Cincotti, Livia Leoni, Paolo Visca
  • Genomic Comparison Reveals Natural Occurrence of Clinically Relevant Multidrug-Resistant Extended-Spectrum-β-Lactamase-Producing <span class="named-content genus-species" id="named-content-1">Escherichia coli</span> Strains
    Food Microbiology | Spotlight
    Genomic Comparison Reveals Natural Occurrence of Clinically Relevant Multidrug-Resistant Extended-Spectrum-β-Lactamase-Producing Escherichia coli Strains

    Multidrug-resistant (MDR) Escherichia coli isolates pose global threats to public health due to the decreasing availability of treatment options. To better understand the characteristics of MDR E. coli isolated from food-producing animals with no antibiotic exposure, we employed genomic comparison,...

    Lin Teng, Shinyoung Lee, Amber Ginn, Sarah M. Markland, Raies A. Mir, Nicolas DiLorenzo, Christina Boucher, Mattia Prosperi, Judith Johnson, J. Glenn Morris, Kwangcheol C. Jeong
  • Open Access
    Public and Environmental Health Microbiology
    Transferable Plasmid-Borne mcr-1 in a Colistin-Resistant Shigella flexneri Isolate
    Beibei Liang, Adam P. Roberts, Xuebin Xu, Chaojie Yang, Xiaoxia Yang, Jinyan Wang, Shengjie Yi, Yongrui Li, Qiuxia Ma, Fuli Wu, Shaofu Qiu, Hongbin Song
  • Public and Environmental Health Microbiology | Spotlight
    Multidrug and Mupirocin Resistance in Environmental Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates from Homes of People Diagnosed with Community-Onset MRSA Infection
    J. H. Shahbazian, P. D. Hahn, S. Ludwig, J. Ferguson, P. Baron, A. Christ, K. Spicer, P. Tolomeo, A. M. Torrie, W. B. Bilker, V. C. Cluzet, B. Hu, K. Julian, I. Nachamkin, S. C. Rankin, D. O. Morris, E. Lautenbach, M. F. Davis
  • Minireview
    The Current State of Macrolide Resistance in Campylobacter spp.: Trends and Impacts of Resistance Mechanisms
    Hannah Bolinger, Sophia Kathariou
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