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nisin

  • Nisin M: a Bioengineered Nisin A Variant That Retains Full Induction Capacity but Has Significantly Reduced Antimicrobial Activity
    Biotechnology | Spotlight
    Nisin M: a Bioengineered Nisin A Variant That Retains Full Induction Capacity but Has Significantly Reduced Antimicrobial Activity

    This study describes the generation of a nisin variant with superior characteristics for use in the NICE protein expression system. The variant, termed nisin M, retains an induction capacity comparable to that of wild-type nisin A but exhibits significantly reduced antimicrobial activity and can therefore be used at concentrations that are normally toxic to the expression host.

    Michelle O’ Connor, Des Field, Aoife Grainger, Paula M. O’ Connor, Lorraine Draper, R. Paul Ross, Colin Hill
  • ABC Transporter DerAB of <span class="named-content genus-species" id="named-content-1">Lactobacillus casei</span> Mediates Resistance against Insect-Derived Defensins
    Environmental Microbiology
    ABC Transporter DerAB of Lactobacillus casei Mediates Resistance against Insect-Derived Defensins

    Antimicrobial peptides (AMPs) play an important role in suppressing the growth of microorganisms. They can be produced by bacteria themselves—to inhibit competitors—but are also widely distributed in higher eukaryotes, including insects and mammals, where they form an important component of innate immunity. In low-GC-content Gram-positive bacteria, BceAB-like transporters play a crucial role in AMP resistance but have so far been...

    Ainhoa Revilla-Guarinos, Qian Zhang, Christoph Loderer, Cristina Alcántara, Ariane Müller, Mohammad Rahnamaeian, Andreas Vilcinskas, Susanne Gebhard, Manuel Zúñiga, Thorsten Mascher
  • <span class="sc">d</span>-Methionine and <span class="sc">d</span>-Phenylalanine Improve <span class="named-content genus-species" id="named-content-1">Lactococcus lactis</span> F44 Acid Resistance and Nisin Yield by Governing Cell Wall Remodeling
    Genetics and Molecular Biology
    d-Methionine and d-Phenylalanine Improve Lactococcus lactis F44 Acid Resistance and Nisin Yield by Governing Cell Wall Remodeling

    As d-amino acids play an important role in cell wall synthesis, we analyzed the effects of 19 different d-amino acids on L. lactis F44, demonstrating that d-Met and d-Phe can participate in peptidoglycan (PG) synthesis and improve the acid resistance and nisin yield of this...

    Hao Wu, Ershu Xue, Ning Zhi, Qianqian Song, Kairen Tian, Qinggele Caiyin, Lin Yuan, Jianjun Qiao
  • LanI-Mediated Lantibiotic Immunity in <span class="named-content genus-species" id="named-content-1">Bacillus subtilis</span>: Functional Analysis
    Environmental Microbiology | Spotlight
    LanI-Mediated Lantibiotic Immunity in Bacillus subtilis: Functional Analysis

    The two lantibiotics nisin and subtilin are produced by Lactococcus lactis and Bacillus subtilis, respectively. Both peptides have strong antimicrobial activity against Gram-positive bacteria, and therefore, appropriate protection mechanisms are required for the producing strains. To prevent...

    Christoph Geiger, Sophie Marianne Korn, Michael Häsler, Oliver Peetz, Janosch Martin, Peter Kötter, Nina Morgner, Karl-Dieter Entian
  • Genetics and Molecular Biology
    Increasing the Antimicrobial Activity of Nisin-Based Lantibiotics against Gram-Negative Pathogens
    Qian Li, Manuel Montalban-Lopez, Oscar P. Kuipers
  • Genetics and Molecular Biology | Spotlight
    Specificity of Subtilin-Mediated Activation of Histidine Kinase SpaK
    Christoph Geiger, Tobias Spieß, Sophie Marianne Korn, Peter Kötter, Karl-Dieter Entian
  • Biotechnology | Spotlight
    Carboxyl Analogue of Mutacin 1140, a Scaffold for Lead Antibacterial Discovery
    Jerome Escano, Akshaya Ravichandran, Bita Salamat, Leif Smith
  • Food Microbiology
    A Multibacteriocin Cheese Starter System, Comprising Nisin and Lacticin 3147 in Lactococcus lactis, in Combination with Plantaricin from Lactobacillus plantarum
    S. Mills, C. Griffin, P. M. O'Connor, L. M. Serrano, W. C. Meijer, C. Hill, R. P. Ross
  • Food Microbiology
    Glutamate Decarboxylase-Mediated Nisin Resistance in Listeria monocytogenes
    Máire Begley, Paul D. Cotter, Colin Hill, R. Paul Ross
  • Physiology
    Synergistic Effects of the Lactobacillus acidophilus Surface Layer and Nisin on Bacterial Growth
    Mariano Prado-Acosta, Sandra M. Ruzal, Mariana C. Allievi, María Mercedes Palomino, Carmen Sanchez Rivas

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