nisin
Biotechnology | SpotlightNisin M: a Bioengineered Nisin A Variant That Retains Full Induction Capacity but Has Significantly Reduced Antimicrobial ActivityThis study describes the generation of a nisin variant with superior characteristics for use in the NICE protein expression system. The variant, termed nisin M, retains an induction capacity comparable to that of wild-type nisin A but exhibits significantly reduced antimicrobial activity and can therefore be used at concentrations that are normally toxic to the expression host.
Environmental MicrobiologyABC Transporter DerAB of Lactobacillus casei Mediates Resistance against Insect-Derived DefensinsAntimicrobial peptides (AMPs) play an important role in suppressing the growth of microorganisms. They can be produced by bacteria themselves—to inhibit competitors—but are also widely distributed in higher eukaryotes, including insects and mammals, where they form an important component of innate immunity. In low-GC-content Gram-positive bacteria, BceAB-like transporters play a crucial role in AMP resistance but have so far been...
Genetics and Molecular Biologyd-Methionine and d-Phenylalanine Improve Lactococcus lactis F44 Acid Resistance and Nisin Yield by Governing Cell Wall RemodelingAs d-amino acids play an important role in cell wall synthesis, we analyzed the effects of 19 different d-amino acids on L. lactis F44, demonstrating that d-Met and d-Phe can participate in peptidoglycan (PG) synthesis and improve the acid resistance and nisin yield of this...
Environmental Microbiology | SpotlightLanI-Mediated Lantibiotic Immunity in Bacillus subtilis: Functional AnalysisThe two lantibiotics nisin and subtilin are produced by Lactococcus lactis and Bacillus subtilis, respectively. Both peptides have strong antimicrobial activity against Gram-positive bacteria, and therefore, appropriate protection mechanisms are required for the producing strains. To prevent...
- Genetics and Molecular BiologyIncreasing the Antimicrobial Activity of Nisin-Based Lantibiotics against Gram-Negative Pathogens
- Genetics and Molecular Biology | SpotlightSpecificity of Subtilin-Mediated Activation of Histidine Kinase SpaK
- Biotechnology | SpotlightCarboxyl Analogue of Mutacin 1140, a Scaffold for Lead Antibacterial Discovery