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nitrogenase

  • Open Access
    Large Hydrogen Isotope Fractionation Distinguishes Nitrogenase-Derived Methane from Other Methane Sources
    Geomicrobiology | Spotlight
    Large Hydrogen Isotope Fractionation Distinguishes Nitrogenase-Derived Methane from Other Methane Sources

    All forms of life require nitrogen for growth. Many different kinds of microbes living in diverse environments make inert nitrogen gas from the atmosphere bioavailable using a special enzyme, nitrogenase. Nitrogenase has a wide substrate range, and, in addition to producing bioavailable nitrogen, some forms of nitrogenase also produce small amounts of the greenhouse gas methane. This is different from other microbes that produce methane...

    Katja E. Luxem, William D. Leavitt, Xinning Zhang
  • Diazotrophic <em>Anaeromyxobacter</em> Isolates from Soils
    Environmental Microbiology
    Diazotrophic Anaeromyxobacter Isolates from Soils

    Anaeromyxobacter is globally distributed in soil environments, especially predominant in paddy soils. Current studies based on environmental DNA/RNA analyses frequently detect gene fragments encoding nitrogenase of Anaeromyxobacter from various soil environments. Although the importance of Anaeromyxobacter as a diazotroph in nature has been suggested by culture-independent studies, there has been no solid...

    Yoko Masuda, Haruka Yamanaka, Zhen-Xing Xu, Yutaka Shiratori, Toshihiro Aono, Seigo Amachi, Keishi Senoo, Hideomi Itoh
  • Influence of Energy and Electron Availability on <em>In Vivo</em> Methane and Hydrogen Production by a Variant Molybdenum Nitrogenase
    Physiology
    Influence of Energy and Electron Availability on In Vivo Methane and Hydrogen Production by a Variant Molybdenum Nitrogenase

    A variant form of Mo nitrogenase catalyzes the conversion of CO2 and protons to the biofuels CH4 and H2. A constant supply of electrons and ATP is needed to drive these reduction reactions. The bacterium R. palustris generates ATP from light and has a versatile metabolism that makes it ideal for manipulating electron availability...

    Yanning Zheng, Caroline S. Harwood
  • Introduction of Glyoxylate Bypass Increases Hydrogen Gas Yield from Acetate and <span class="sc">l</span>-Glutamate in <em>Rhodobacter sphaeroides</em>
    Biotechnology
    Introduction of Glyoxylate Bypass Increases Hydrogen Gas Yield from Acetate and l-Glutamate in Rhodobacter sphaeroides

    As an alternative to fossil fuel, H2 is a promising renewable energy source. Although photofermentative H2 production from acetate is key to developing an efficient process of biohydrogen production from biomass-derived sugars, H2 yields from acetate and l-glutamate by R. sphaeroides have been reported to be low...

    Tetsu Shimizu, Haruhiko Teramoto, Masayuki Inui
  • Biotechnology
    Transcriptional Analysis of an Ammonium-Excreting Strain of Azotobacter vinelandii Deregulated for Nitrogen Fixation
    Brett M. Barney, Mary H. Plunkett, Velmurugan Natarajan, Florence Mus, Carolann M. Knutson, John W. Peters
  • Environmental Microbiology
    Detection of Diazotrophy in the Acetylene-Fermenting Anaerobe Pelobacter sp. Strain SFB93
    Denise M. Akob, Shaun M. Baesman, John M. Sutton, Janna L. Fierst, Adam C. Mumford, Yesha Shrestha, Amisha T. Poret-Peterson, Stacy Bennett, Darren S. Dunlap, Karl B. Haase, Ronald S. Oremland
  • Enzymology and Protein Engineering
    Site-Directed Mutagenesis of the Anabaena sp. Strain PCC 7120 Nitrogenase Active Site To Increase Photobiological Hydrogen Production
    Hajime Masukawa, Kazuhito Inoue, Hidehiro Sakurai, C. Peter Wolk, Robert P. Hausinger
  • Environmental Microbiology
    Characterization of Diazotrophs Containing Mo-Independent Nitrogenases, Isolated from Diverse Natural Environments
    Doris A. Betancourt, Telisa M. Loveless, James W. Brown, Paul E. Bishop
  • PHYSIOLOGY AND BIOTECHNOLOGY
    Effects of Disruption of Homocitrate Synthase Genes on Nostoc sp. Strain PCC 7120 Photobiological Hydrogen Production and Nitrogenase
    Hajime Masukawa, Kazuhito Inoue, Hidehiro Sakurai
  • PHYSIOLOGY AND BIOTECHNOLOGY
    Redirection of Metabolism for Biological Hydrogen Production
    Federico E. Rey, Erin K. Heiniger, Caroline S. Harwood

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