nonribosomal peptide synthetase
BiotechnologySynthesis of d-Amino Acid-Containing Dipeptides Using the Adenylation Domains of Nonribosomal Peptide SynthetaseBecause almost all amino acids in nature are l-amino acids, the functioning of d-amino acids has received little attention. Thus, there is little information available on the activity of enzymes toward d-amino acids or synthetic methods for d-amino acid-containing dipeptides. Recently, d-amino acids and...
Genetics and Molecular BiologyAlternative Biosynthetic Starter Units Enhance the Structural Diversity of Cyanobacterial LipopeptidesHerein, we deciphered the most important biosynthetic traits of a prominent group of bioactive lipopeptides. We reveal evidence for initiation of biosynthesis by two alternative starter units hardwired directly in the same gene cluster, eventually resulting in the production of a remarkable range of lipopeptide variants. We identified several unusual tailoring genes potentially involved in modifying the fatty acid chain. Careful...
- Enzymology and Protein Engineering | SpotlightA Highly Conserved Basidiomycete Peptide Synthetase Produces a Trimeric Hydroxamate Siderophore