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Applied and Environmental Microbiology
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Streptococcus agalactiae

  • Methods
    Stable Expression of Modified Green Fluorescent Protein in Group B Streptococci To Enable Visualization in Experimental Systems

    Group B streptococcus (GBS) is a bacterium associated with various diseases in humans and animals. This study describes the development of a strategy to label and visualize GBS using a fluorescence biomarker, termed GFPmut3. We show that this biomarker can be successfully applied to track the growth of bacteria in liquid medium, and it enables the detailed visualization of GBS in the context of live human cells in real-time microscopic...

    Matthew J. Sullivan, Glen C. Ulett
  • Evolutionary and Genomic Microbiology
    Microevolution of Streptococcus agalactiae ST-261 from Australia Indicates Dissemination via Imported Tilapia and Ongoing Adaptation to Marine Hosts or Environment

    Streptococcus agalactiae (GBS) is a significant pathogen of humans and animals. Some lineages have become adapted to particular hosts, and serotype Ib is highly specialized to fish. Here, we show that this lineage is likely to have been distributed widely by the global trade in tilapia for aquaculture, with probable introduction into Australia in the 1970s and...

    Minami Kawasaki, Jerome Delamare-Deboutteville, Rachel O. Bowater, Mark J. Walker, Scott Beatson, Nouri L. Ben Zakour, Andrew C. Barnes
  • Enzymology and Protein Engineering
    LambdaSa1 and LambdaSa2 Prophage Lysins of Streptococcus agalactiae
    David G. Pritchard, Shengli Dong, Marion C. Kirk, Robert T. Cartee, John R. Baker
  • Evolutionary and Genomic Microbiology
    Mobile Genetic Elements Provide Evidence for a Bovine Origin of Clonal Complex 17 of Streptococcus agalactiae
    Geneviève Héry-Arnaud, Guillaume Bruant, Philippe Lanotte, Stella Brun, Bertrand Picard, Agnès Rosenau, Nathalie van der Mee-Marquet, Pascal Rainard, Roland Quentin, Laurent Mereghetti
  • PHYSIOLOGY AND BIOTECHNOLOGY
    Granadaene: Proposed Structure of the Group B Streptococcus Polyenic Pigment
    Manuel Rosa-Fraile, Javier Rodríguez-Granger, Ali Haidour-Benamin, Juan Manuel Cuerva, Antonio Sampedro
  • PHYSIOLOGY AND BIOTECHNOLOGY
    The Cell Lysis Activity of the Streptococcus agalactiae Bacteriophage B30 Endolysin Relies on the Cysteine, Histidine-Dependent Amidohydrolase/Peptidase Domain
    David M. Donovan, Juli Foster-Frey, Shengli Dong, Geneviève M. Rousseau, Sylvain Moineau, David G. Pritchard
  • PHYSIOLOGY AND BIOTECHNOLOGY
    Peptidoglycan Hydrolase Fusions Maintain Their Parental Specificities
    David M. Donovan, Shengli Dong, Wes Garrett, Geneviève M. Rousseau, Sylvain Moineau, David G. Pritchard
  • Methods
    Use of glnQ as a Counterselectable Marker for Creation of Allelic Exchange Mutations in Group B Streptococci
    Glen S. Tamura, Debra S. Bratt, Harry H. Yim, Aphakorn Nittayajarn
  • PHYSIOLOGY AND BIOTECHNOLOGY
    Pigment Production by Streptococcus agalactiae in Quasi-Defined Media
    Manuel Rosa-Fraile, Antonio Sampedro, Javier Rodrı́guez-Granger, Maria Luisa Garcı́a-Peña, Alfonso Ruiz-Bravo, Ali Haı̈dour
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