whole-genome sequencing
Public and Environmental Health MicrobiologyEffective Surveillance Using Multilocus Variable-Number Tandem-Repeat Analysis and Whole-Genome Sequencing for Enterohemorrhagic Escherichia coli O157Intensive surveillance for enterohemorrhagic Escherichia coli (EHEC) serogroup O157 is important to detect outbreaks and to prevent the spread of the bacterium. Recent advances in sequencing technology made molecular surveillance using whole-genome sequence (WGS) realistic. To develop rapid, high-throughput, and cost-effective typing methods for real-time surveillance...
Public and Environmental Health Microbiology | SpotlightIn Silico Serotyping Based on Whole-Genome Sequencing Improves the Accuracy of Shigella IdentificationShigella causes diarrheal disease with serious public health implications. However, conventional Shigella identification methods are laborious and time-consuming and can be erroneous due to the high similarity between Shigella and enteroinvasive Escherichia coli (EIEC) and cross-reactivity between serotyping antisera. Further, serotype...
Public and Environmental Health MicrobiologyPopulation Genetics and Characterization of Campylobacter jejuni Isolates from Western Jackdaws and Game Birds in FinlandThe roles of environmental reservoirs, including wild birds, in the molecular epidemiology of Campylobacter jejuni have not been assessed in depth. Our results showed that game birds may pose a risk for acquiring campylobacteriosis, because they had C. jejuni genomotypes highly similar to human...
- Public and Environmental Health MicrobiologyYersinia pseudotuberculosis Prevalence and Diversity in Wild Boars in Northeast Germany
Yersinia pseudotuberculosis is a foodborne pathogen whose occurrence is poorly understood. One reason for this situation is the difficulty in isolating the species. The methods developed for the isolation of Yersinia enterocolitica are not well suited for...
- Public and Environmental Health MicrobiologyGenome Sequencing Links Persistent Outbreak of Legionellosis in Sydney (New South Wales, Australia) to an Emerging Clone of Legionella pneumophila Sequence Type 211