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BiotechnologyHomoethanol Production from Glycerol and Gluconate Using Recombinant Klebsiella oxytoca StrainsGluconate is a biomass-derived degradation product, and glycerol can be obtained as a biodiesel byproduct. Compared to glucose, using them as the sole substrate is accompanied by the production of by-products. Our study shows that through pathway engineering and adoption of a fed-batch culture system, high-yield homoethanol production that usually can be achieved by using glucose as the substrate is achievable using gluconate and...
Environmental MicrobiologyBacillus velezensis Wall Teichoic Acids Are Required for Biofilm Formation and Root ColonizationBacillus velezensis is a Gram-positive plant-beneficial bacterium which is widely used in agriculture. Additionally, Bacillus spp. are some of the model organisms used in the study of biofilms, and as such, the molecular networks and regulation systems of biofilm formation are well characterized....
Enzymology and Protein Engineering | SpotlightActivity and Thermostability of GH5 Endoglucanase Chimeras from Mesophilic and Thermophilic ParentsThermal stability is an essential property of enzymes in many industrial biotechnological applications, as high temperatures improve bioreactor throughput. Many protein engineering approaches, such as rational design and directed evolution, have been employed to improve the thermal properties of mesophilic enzymes. Structure-based recombination has also been used to fuse TIM barrel fragments, and even fragments from unrelated folds, to...
- Enzymology and Protein EngineeringStructure-function analysis indicates that an active site water participates in dimethylsulfoniopropionate cleavage by DddK
- Environmental MicrobiologyKey physiology of a nitrite-dependent methane-oxidizing enrichment culture
- Genetics and Molecular BiologyThe Edwardsiella piscicida type III translocon protein EseC inhibits biofilm formation through sequestering EseE
- Public and Environmental Health MicrobiologyDisruption of quorum sensing and virulence in Burkholderia cenocepacia by a structural analogue of the cis-2-dodecenoic acid signal
